Effect of Overexpression of the Brain-Specific Na+/Ca2+ Exchanger Splice Variant NCX1.5 on NO Cytotoxicity in HEK293 Cells

Effect of Overexpression of the Brain-Specific Na+/Ca2+ Exchanger Splice Variant NCX1.5 on NO Cytotoxicity in HEK293 Cells

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Abstract.: Nitric oxide (NO) induces cytotoxicity in neuronal and glial cells via activation of the Na+/Ca2+ exchanger (NCX).This study examined the role of the predominant brain-specific NCX splice wac 4011 variant NCX1.5 in NO-induced cytotoxicity in the HEK293 cell expression system.

Cells were transfected with the plasmid construct pcDNA3.1/V5-His containing full-length rat NCX1.5 cDNA.There was no difference in the altitude sunscreen cytotoxic effects of the NO donors sodium nitroprusside and S-nitroso-N-acetylpenicillamine between control and transfected cells.

These results suggest that NO cytotoxicity is not dependent on NCX1.5.Keywords:: Na+/Ca2+ exchanger, nitric oxide, cytotoxicity.